Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
نویسندگان
چکیده
منابع مشابه
Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining.
Periodic acid Schiff (PAS) staining is an immunohistochemical technique used on muscle biopsies and as a diagnostic tool for blood samples. Polysaccharides such as glycogen, glycoproteins, and glycolipids stain bright magenta making it easy to enumerate positive and negative cells within the tissue. In muscle cells PAS staining is used to determine the glycogen content in different types of mus...
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متن کاملStaining of blood cells with periodic acid/salicyloyl hydrazide (PA-SH). A fluorescent method for demonstrating glycogen.
By J. BURNS AND P. B. NEAME T lIE PERIODIC ACID-SCHIFF (PAS ) REACTION is generally accepted as the method of choice for demonstrating glycogen in blood cells. This reaction depends upon the presence, in glycogen, of vicinal glycols which are oxidized by periodic acid to dialdehydes. The latter are subsequently condensed with Schiff’s reagent to produce a magenta-red product, visible by light m...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/52199